Green Spring Malachite green ELISA kit - LSY-10027

Malachite green ELISA kit
1. Principle
This test kit is based on the competitive enzyme immunoassay. The coupling antigen is pre-coated
on the micro-well stripes. The Malachite green in the sample and the coupling antigens pre-coated
on the micro-well stripes compete for the anti-Malachite green antibodies. After the addition of the
enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the
sample has a negative correlation with the Malachite green in it. This value is compared to the
standard curve and the content of Malachite green residues is subsequently obtained.
2. Technical specifications
Sensitivity: 0.1 ppb
Detection limit
Shrimp, Fish............................................................................................. 0.5 ppb
Water........................................................................................................ 0.1 ppb
Scientific metabolism principle and lots of screening positive sample statistics indicate: the
Malachite green total residues of Malachite green and Leucomalachite green in the positive
sample is equal to the Malachite green residues in the testing positive sample multiplied by 2.27.
The total residues of Malachite green and Leucomalachite green (Yppb)= The testing Malachite
green(Xppb)×2.27
Recovery rate
Shrimp, Fish............................................................................................ 90±15%
Cross-reaction rate
Malachite green...........................................................................................100%
Leucomalachite green..................................................................................< 1%
Gentian violet ............................................................................................... 91%
Leucocrystal violet........................................................................................< 1%
3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 1× standard solution (0.4 mL)..............................................................100 ppb
3） Enzyme conjugate (25×) (0.8 mL).....................................................white cap
4） Antibody working solution (9 mL)...................................................... white cap
5） Substrate solution (2 bottle, 16 mL).................................................. black cap
6） Stop solution (12 mL).............................................................. transparent cap
7） 5× concentrated washing buffer (2 bottle, 100 mL)...........................white cap
8） Enzyme conjugate diluent (15 mL)..........................................transparent cap
9） Sample diluent (50 mL)........................................................... transparent cap