Florfenicol ELISA Test Kit - LSY-10008

Florfenicol ELISA Test Kit
Catalog No. LSY-10008
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Florfenicol in the tissue (chicken, pork), fish, shrimp and honey. The coupling antigens are pre-coated on the micro-well stripes. The Florfenicol in the sample and the conjugate antigens pre-coated on the micro-well stripes compete for the anti-Florfenicol antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value has a negative correlation with the Florfenicol concentration in the sample. This value is compared to the standard curve and the Florfenicol concentration is subsequently obtained.

2. Technical specifications
Sensitivity: 0.5 ppb
Incubator temperature: 25℃
Incubator time: 60min～30min～30min
Detection limit:
Honey, tissue 0.5 ppb
Recovery rate:
Tissue 80 �18%
Honey 70 �14%
Cross-reaction rate:
Florfenicol 100%
Florfenicol Amine 11%
Thiamphenicol < 0.1%
Chloramphenicol < 0.1%

3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6� standard solution (1 mL each): 0 ppb, 0.5 ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb and 40.5 ppb
3） Enzyme conjugate (12 mL) red cap
4） Concentrated Antibody working solution (1 mL) blue cap
5） Substrate A solution (7 mL) white cap
6） Substrate B solution (7 mL) black cap
7） Stop solution (7 mL) yellow cap
8） 20� concentrated washing buffer (40 mL) white cap
9） 2� concentrated redissolving solution (50 mL) transparent cap