Ampicillin ELISA Assay Kit

Ampicillin ELISA Test Kit 1. Principle This test kit is based on the competitive enzyme immunoassay for the detection of Ampicillin in the sample. The antigens conjugated Ampicillin is pre-coated on the micro-well stripes, Ampicillin in the sample and the conjugated antigens pre-coated on the micro-well stripes compete for the anti- Ampicillin antibodies.

After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Ampicillin concentration in the sample. This value is compared to the standard curve and concentration of Ampicillin in the sample is subsequently obtained. 2.

Technical specifications Sensitivity : 0.1 ppb Detection limit: Chicken, duck, pork,liver, fish, shrimp.......................................................2 ppb Honey, milk................................................................................................ 2 ppb Because of some interference in honey and milk, the detection limit is 4 ppb.

Recovery rate: Chicken, duck, pork, liver, fish, shrimp.............................................. 75%±10% Honey, milk.........................................................................................70%±10% Cross-reaction rate: Ampicillin...................................................................................................

100% Benzyl penicillin..........................................................................................0.8% Cloxacillin...................................................................................................

0.2% Dicloxacillin.................................................................................................0.1% Amoxicillin.................................................................................................. 0.1% Ceftiofu....................................................................................................... 0.1% 3.

Components 1） Micro-well strips: 12 strips with 8 removable wells each 2） 6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7ppb and 8.1 ppb 3） Enzyme conjugate (12 mL)...................................................................red cap 4） Antibody working solution (7 mL)........................................................ blue cap 5） Substrate A solution (7 mL)................................................................white cap 6） Substrate B solution (7 mL)............................................................... black cap 7） Stop solution (7 mL)......................................................................... yellow cap 8） 20× concentrated washing buffer (40 mL).........................................white cap 9） 5× concentrated redissolving solution (50 mL)........................transparent cap