Subtilisin

枯草杆菌蛋白酶 Subtilisin/Subtilisin from bacillus subtilis

Synonyms: Subtilisin Carlsberg, Subtilopeptidase A, Bacterial Alkaline Protease

CAS #: 9014-01-1

EC #: 3.4.21.62

Sigma's Subtilisins are produced from fermentation of Bacillus licheniformis. We offer several grades and formulations including high purity lyophilized powders as well as economical industrial grade solutions.

Physical properties and Specificity

MW: Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and a of MW 27 KDa.2

Specificity: Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.

Isoelectric point: pI = 9.4

E1%(280nm) = 8.6 Sedimentation coefficient: S20,w = 2.773

Activity Determination and Optimal Conditions

Unit definition: One Sigma Unit will hydrolyze casein to produce color equivalent to 1.0 mmole (181 µg) of tyrosine per minute at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Applications

Research tool

In molecular biology using B. subtilis as a model organism, the gene encoding subtilisin (aprE) is often the second gene of choice after amyE for integrating reporter constructs into, due to its dispensability.

Commercial

Protein-engineered subtilisins are widely used in commercial products (the native enzyme is easily inactivated by detergents and high temperatures) and is also called a stain cutter, for example, in laundry and dishwashing detergents, cosmetics, food processing, skin care ointments, contact lens cleaners, and for research in synthetic organic chemistry.