PCR Master Mix

2XTaq PCR MasterMix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, PCR enhancer and all the components required for PCR, except DNA template and primers. The Mix can significantly enhance the PCR amplification Sensitivity and specificity.The pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The amplified PCR products can be directly applied to T/A cloning. The Mix can amplify fragment up to 5kb with low GC and also can amplify GC-rich template (more than 80%).The MasterMix contains a dye and precipitant to facilitate gel loading.

Features

Convenient - in a ready-to-use mix.

Fast -saves time due to reduced number of pipetting steps.

Reproducible -lower contamination and pipetting error risk.

Compatible with TA cloning - generates PCR products with 3'-A overhangs.

Composition of 2X PCR Master Mix

·0.05u/µl Taq DNA Polymerase (recombinant)

·reaction buffer

·4mM MgCl2

·0.4mM of each dNTP (dATP, dCTP, dGTP, dTTP).

Applications

·High throughput PCR.

·Routine PCR with high reproducibility.

·Generation of PCR products for TA cloning.

·RT-PCR.