Nitroimidazole ELISA Diagnostic Kit

Nitroimidazole is an antibacterial agent for the treatment and prevention of trichomoniasis, amoeba and anaerobic infections. Because of being carcinogenic, many countries prohibit it for breeding livestock, poultry and aquatic products. It is prohibited to use as animal growth-promoting agents.

Nitroimidazole ELISA kits utilizing the latest biotechnology development has easy,rapid,and sensitive advantages. This kit uses indirect-competition method mainly based on the immunological reaction between specific antigen and antibody.

Nitroimidazole specific antigen coated on microtiter plates competes binding sites of enzyme-labeled Nitroimidazole specific antibody with added calibrator (or sample). With TMB chromogenic substrate, the absorbance value was negatively correlated with residues of Nitroimidazole.

Under the calibration curve, Nitroimidazole content of the sample can be quantified multiplied by the corresponding compare dilution.

Specificity Metronidazole -------------------------------------------------100% Hydroxy metronidazole -------------------------------------- 47% Hydroxymethyl metronidazole imidazole ---------------- 53% Tinidazole --------------------------------------------------------- 2% Dimetridazole ------------------------------------------------- 800% Ornidazole -------------------------------------------------------- 5% Detection limit: 0.1ppb is the lowest detection limit of Nitroimidazole kit.

Linear regression is between 0.1-8.1ppb.

Sensitivity: Tissue ---------------------------------------------------0.5ppb Honey ---------------------------------------------------0.1ppb Milk -------------------------------------------------------0.2ppb Recovery rate: Tissue ------------------------------------------------60-120% Honey ------------------------------------------------60-120% Milk------------------------------------------------60-120%