Chlortetracycline ELISA Diagnostic Kit

EU preliminary provisions total muscle and milk tetracycline compounds shall not exceed 100ppb, in the kidney, liver and eggs respectively shall not exceed 600 ppb, 300 ppb and 200ppb. The Chl- ortetracycline ELISA kits utilizing the latest biotechnology development has easy, rapid, and sensitive advantages.

This kit uses indirect-competition method mainly based on the immunological reaction between specific antigen and antibody. Chlortetracycline specific antigen coated on microtiter plates competes binding sites of enzyme-labeled Chlortetracycline specific antibody with added calibrator (or sample).

With TMB chromogenic substrate, the absorbance value was negatively correlated with residues of Chlortetracycline. Under the calibration curve,Chlortetracycline content of the sample can be quantified multiplied by the corresponding compare dilution.

Specificity Chlortetracycline----------------------------------------------100% Tetracycline----------------------------------------------------100% Oxytetracycline------------------------------------------------50% doxycycline (doxycycline) -----------------------------------10% Detection limit: 0.4ppb is the lowest detection limit of Chlortetracycline kit.

Linear regression is between 0.4-10.8ppb. Sensitivity: Tissue ------------------------------------------------------6ppb Recovery rate: Tissue--------------------------------------------------75-105%