Bupleurum Extract Bupleurum saikosaponins

Product Specification: 5% Bupleurum saikosaponins Test method: HPLC/UV Chemical Name: 5(hydroxymethyl)2furfural Raw material: Umbelliferaer Bupleurum Extract Method: Water Product Type: Brown yellow CAS#: [20874-52-6] 1.

Physical and Chemical Properties Molecular structure of type: Saponins compounds Molecular formula and weight: C20H16N2O5 Melt Point: 225～232℃ Solubility: Good soluble in water 2.

Quality standards testing 2.1 Identification: (reference substance: Bupleurum Extract and saikosaponins) 2.1.2 TLC examination: reference substance Rf value and color are consistency 2.1.3 IR inspection: in line with the reference substance fingerprint (infrared absorption spectra) 2.2 HPLC Chromatogram of Bupleurum extract 98% 2.2.1 Theory Detection the concentration of resvertration in the samples by using a standard working curve 2.2.2 Reagents and equipment Chromatography grade methanol High Performance Liquid Chromatogram equipment Ultrasonic equipment 2.2.3 Liquid sample configuration Accurately take 5mg of 5% Bupleurum Extract samples, dissolved with appropriate chromatography grade methanol, Calibration volume to 10mL, ultrasound 30mins and laying 10min, using 0.45μm membrane filtrate, as the sample liquid reserve.

2.2.4 Preparation of standard solution Accurately take 100mg Bupleurum extract standard, dissolved with chromatography grade methanol and calibrate volume to 10mL.

2.2.5 Detect conditions: Chromatography Column: KYWG, C18(250×4)mm, 5μm Mobile phase: 27% acetonitrile aqueous solution Velocity: 1.2 mL/min Test wave: 303 nm 2.2.6 Standard curve Accurately take 0.20mL, 0.15mL, 0.1mL, 0.05mL, 0.00mL to 10mL flask, Calibrate volume respectively, made concentration of 1mg/mL, 0.75mg/mL, 0.5mg/mL, 0.25 mg/mL, and 0.00mg/mL solution of the reference substance.

Using the method of 2.2.5 to detect those solutions, the concentration as the vertical coordinates and the peak area as Abscissa, rendering the standard curve, and elicit to the regression equation. 2.2.7 Sample content test Detect the sample liquid as 2.2.5’s method; calculate the peak area and its content by the standard working curve.

HPLC Bupleurum Extract of 5% saikosaponins 2.3 Examine Loss on Drying: NMT 2% Ash: NMT 0.5% Heavy Metals: NMT 5 ppm Pesticide Residues: NMT 2 ppm Solvents Residues: NMT 0.1% 2.3 Hygiene inspection Total bacteria: NMT 1000/g Total Streptomyces: NMT 100/g E.Coli: None Salmonella: None 2.4 Sieve Analysis: 100% pass 80mesh 2.5Packing: Packed in paper-drums and two plastic-bags inside.

Barrels outside printed name, gross weight, batch number, production units, production time, effective time, storage and transportation methods. 2.6 Effective time: two years 2.7 Storage situation: Stored in a cool and dry well-closed container, keep away from moisture and strong light / heat.