Rosemary Herb Extract - vanessa@nutra-max.com

Product Specification:5%~98%
Test method: HPLC/UV
Effective ingredients：Carnosic Acid，Rosmarinic acid Raw material: leaf
Extract Method: Grain Alcohol
1. Physical and Chemical Properties
Molecular structure of type: Polyphenol compounds
Melt Point: 253℃～257℃
Solubility: Difficult to dissolve in water, soluble in ethanol, acetone and other organic solvents
2. Quality standards testing
2.1 Identification:
2.1.2 TLC examination: reference substance Rf value and color are consistency
2.1.3 IR inspection: in line with the reference substance fingerprint (infrared absorption spectra)
2.2 HPLC Chromatogram of 15%Rosmarinic acid
2.2.1 Theory
Detection the concentration of resvertration in the samples by using a standard working curve
2.2.2 Reagents and equipment
Chromatography grade methanol
High Performance Liquid Chromatogram equipment
Ultrasonic equipment
2.2.3 Liquid sample configuration
Accurately take 5mg of 98% Rosmarinic acid samples, dissolved with appropriate chromatography grade methanol, Calibration volume to 10mL, ultrasound 30mins and laying 10min, using 0.45μm membrane filtrate, as the sample liquid reserve.
2.2.4 Preparation of standard solution
Accurately take 100mg resveratrol standard, dissolved with chromatography grade methanol and calibrate volume to 10mL.
2.2.5 Detect conditions:
Chromatography Column: KYWG, C18(250×4)mm, 5μm
Mobile phase: 27% acetonitrile aqueous solution
Velocity: 1.2 mL/min
Test wave: 303 nm
2.2.6 Standard curve
Accurately take 0.20mL, 0.15mL, 0.1mL, 0.05mL, 0.00mL to 10mL flask, Calibrate volume respectively, made concentration of 1mg/mL, 0.75mg/mL, 0.5mg/mL, 0.25 mg/mL, and 0.00mg/mL solution of the reference substance. Using the method of 2.2.5 to detect those solutions, the concentration as the vertical coordinates and the peak area as Abscissa, rendering the standard curve, and elicit to the regression equation.
2.2.7 Sample content test
Detect the sample liquid as 2.2.5’s method; calculate the peak area and its content by the standard working curve.



2.3 Examine
Loss on Drying: NMT 2%
Ash: NMT 0.5%
Heavy Metals: NMT 5 ppm
Pesticide Residues: NMT 2 ppm
Solvents Residues: NMT 0.1%
2.3 Hygiene inspection
Total bacteria: NMT 1000/g
Total Streptomyces: NMT 100/g
E.Coli: None
Salmonella: None
2.4 Sieve Analysis: 100% pass 80mesh2.5 Packing: Packed in paper-drums and two plastic-bags inside. Barrels outside printed name, gross weight, batch number, production units, production time, effective time, storage and transportation methods.2.6 Effective time: two years2.7 Storage situation: Stored in a cool and dry well-closed container, keep away from moisture and strong light / heat.3. Extraction Process Flow Diagram

Product Type: yellow or light yellow powder