Bitter Sophora Extract（vanessa@nutra-max.com） - vanessa@nutra-max.com

Bitter Sophora Root Extract
Product Specification: 98% Matrine / 98% Oxymatrine
Test method: HPLC
Chemical Name: Matrine, Oxymatrine
Raw material: Root of Sophora flavescens
Extract Method: Grain Alcohol
Product Type: Light yellow powder
CAS#: Matrine 519-02-8
Oxymatrine 16837-52-8

1. Physical and Chemical Properties
Molecular structure of type: Alkaloids compounds
Molecular formula and weight:
Matrine: C15H24N2O 248.37
Oxymatrine: C15H24N2O2.H2O 264.36
Solubility: Both can dissolve in water, benzene, chloroform, methanol and ethanol
2. Quality standards testing
2.1 Identification: (reference substance: Bitter Sophora Root Extract)
2.1.2 TLC examination: reference substance Rf value and color are consistency
2.1.3 IR inspection: in line with the reference substance fingerprint (infrared absorption spectra)
2.2 HPLC Chromatogram of Matrine and Oxymatrine in Bitter Sophora Root Extract
2.2.1 Theory
Detection the concentration of Matrine and Oxymatrine in Bitter Sophora Root Extract by HPLC
2.2.2 Reagents and equipment
Chromatography grade acetonitrile
Phosphoric acid
Chromatography grade ethanol
High Performance Liquid Chromatogram equipment
Ultrasonic equipment
2.2.3 Test sample configuration
Accurately take Bitter Sophora root extract samples, dissolved with 30mL Mobile phase ultrasonic 30min, put it aside until room temperature, calibrate volume with mobile phase, filtered by 0.45μm membrane.
2.2.4 Preparation of standard solution
Accurately take 5mg Matrine and Oxymatrine standard sample, dissolved with Mobile phaseand calibrate volume to 50mL.
2.2.5 Detect conditions:
Chromatography Column: ODS, C18(250×4.6)mm, 5μm
Mobile phase: Acetonitrile: phosphoric acid aqueous solution (PH: 2.0): ethanol = 80:10: 8
Velocity: 1.0 mL/min
Test wave: 220 nm
2.2.6 Sample content test
Detect the sample liquid as 2.2.5’s method.
2.3 Examine
Loss on Drying: NMT 2%
Ash: NMT 0.5%
Heavy Metals: NMT 5 ppm
Pesticide Residues: NMT 2 ppm
Solvents Residues: NMT 0.1%
2.3 Hygiene inspection
Total bacteria: NMT 1000/g
Total Streptomyces: NMT 100/g
E.Coli: None
Salmonella: None
2.4 Sieve Analysis: 100% pass 80mesh2.5 Packing: Packed in paper-drums and two plastic-bags inside. Barrels outside printed name, gross weight, batch number, production units, production time, effective time, storage and transportation methods.2.6 Effective time: two years2.7 Storage situation: Stored in a cool and dry well-closed container, keep away from moisture and strong light / heat.